tal effector kit 1 0 (Addgene inc)

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Addgene inc tal effector kit 1 0
Tal Effector Kit 1 0, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tal+effector+kit+1+0/Voytas+Lab+-+Golden+Gate+TALEN+and+TAL+Effector+Kit+1%2E0+(Kit+%23+Discontinued)/pmc06023846-223-10-24
Average 93 stars, based on 4 article reviews

tal effector kit 1 0 - by Bioz Stars, 2026-10

93/100 stars

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TALENs:

Article Title: A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models
Article Snippet: .. TALENs were assembled as published [ ], by following the protocol associated with the GoldenGate TALEN and TAL Effector Kit 1.0 (Addgene #1000000016), except that TALE repeats were ultimately cloned into vectors pTAL7A (for TALEN A) and pTAL7B (for TALEN B) (Additional file : Figure S1). .. TALENs A and B were transfected into HEK-293 T cells or hPSCs at equimolar ratios using Fugene 6 (Roche).

Clone Assay:

Article Title: One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors
Article Snippet: .. TAL repeats were assembled by the Golden Gate TALEN and TAL Effector Kit 1.0 supplied by the Daniel Voytas laboratory (no longer available at Addgene, Cambridge, MA) and cloned into the TALEN expression cassette of pCS2TAL3DD or pCS2TAL3RR, which were before cloned into the multiple cloning site of a pBluescript-based vector. ..

Expressing:

Cloning:

Plasmid Preparation:

Article Title: DICE, an efficient system for iterative genomic editing in human pluripotent stem cells
Article Snippet: .. TALEN-encoding plasmids were assembled using the Golden Gate TALEN and TAL Effector Kit 1.0 and its protocol for assembly of the TALEN-encoding plasmid (Addgene; www.addgene.org ). ..

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